Acute and subacute toxicity assessment of liquid CO2 extract of Phaleria macrocarpa fruits flesh in mice model

Phaleria macrocarpa (Scheff.) Boerl., an evergreen tree is found in Indonesia, Malaysia and Oceania, explicitly Northern Territory of Australia Papua New Guinea. The fruits are traditionally used to treat various ailments including diabetes, psoriasis, looseness the bowels, skin inflammation, malignancy, kidney, liver heart sicknesses. However, date, no comprehensive study investigating its toxicity profile utilizing liquid CO 2 extract (LCE) has been reported ICR mice. Hence, this research was performed investigate both acute sub-acute toxicities carbon dioxide from ripe fruit flesh P. Acute assessed at a single dose 3000 mg/kg bw for weeks according up down method OECD 425 guideline, while test by doses 250, 500, 1000, 2000 28 days. animals’ general behaviour, food intake, body weight, organ coefficients, biochemical histological morphology were observed analysed. After exposure LCE, there evidence any severe or fatal effects An result exhibited that LD 50 LCE > bw. findings subacute evaluation revealed 250 500 had significant detrimental impact. Moreover, 1000 demonstrated heart, liver, kidney lung mice identified investigation. results confirmed dosage considered safe can provide better therapeutic effect without eliciting adverse side effects.